Target
Human LIMK2
Synonyms
LIMK2 | LIM domain kinase 2 | LIM kinase 2 | LIMK-2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QVKEVNRMHISPN, from the internal region of the protein sequence according to NP_001026971.1NP_005560.1NP_057952.1.
Specificity
This antibody is expected to recognize all reported isoforms (NP_001026971.1; NP_005560.1; NP_057952.1).
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 75+80kDa doublet band observed in lysates of cell lines HeLa and NIH3T3 and approx 70kDa observed in Rat Pancreas lysates (calculated MW of 77.9kDa according to Human NP_001026971.1 and 72.2kDa according to Human NP_005560.1, Mouse NP_034848.1 and Rat NP_077049.2). Recommended concentration:0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee