LIF antibody LS-C186538 is an unconjugated goat polyclonal antibody to LIF from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Bovine, Dog, Guinea pig, Pig (at least 90% immunogen sequence identity)
- Western blot (0.5 - 1.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
LIF antibody was raised against synthetic peptide TPVNATCAIRHP from an internal region of human LIF (NP_002300.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat (100%); Ferret, Panda, Dog, Cat, Bovine, Pig, Guinea pig (92%); Horse (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186538. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 23kD band observed in Mouse and Rat Colon lysates (calculated MW of 22.3kD according to Mouse NP_032527.1. Recommended concentration: 0.5-1.5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.