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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LIF Antibody (aa24‑203, clone 39N7D10) LS‑C359469
LIF antibody LS-C359469 is an unconjugated rat monoclonal antibody to LIF from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C359469-100
100 µg (1 mg/ml)
$335

Popular LIF Products

Antibody
Species: Human
Applications: Western blot
Anti-LIF antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7078 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
Applications: Western blot
Immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) human prostate using LIF antibody at dilution of 1:100 (40x magnification).
Species: Human
Applications: IHC, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA

Product Description

LIF antibody LS-C359469 is an unconjugated rat monoclonal antibody to LIF from human and mouse. Validated for IHC and WB.
About LIF
Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. P15018 NM_002309 NP_002300.1

LIF Antibody, DIA Antibody, Emfilermin Antibody, HILDA Antibody, Melanoma-derived LPL inhibitor Antibody, MLPLI Antibody, Leukemia inhibitory factor Antibody, CDF Antibody, D factor Antibody, Human interleukin in DA cells Antibody


Specifications

Target
Mouse LIF
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [39N7D10]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (3 - 5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LIF antibody was raised against a recombinant murine Lif protein containing amino acids 24-203.
Epitope
aa24-203
Specificity
Mouse LIF
Usage
The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target).
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Very intense immunopositivity of LIF was observed in membranes as well as the cytoplasm of cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunopositivity of LIF was observed in the cuboidal epithelial cells of renal tubules. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/22/2018

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