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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LHX1 Antibody (aa149‑165) LS‑C737
LHX1 antibody LS-C737 is an unconjugated rabbit polyclonal antibody to human LHX1. Validated for WB.
Catalog
Size
Price
LS-C737-200
200 µl
$335
Description
LHX1 antibody LS-C737 is an unconjugated rabbit polyclonal antibody to human LHX1. Validated for WB.
Target
Human LHX1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
Immunogen
LHX1 antibody was raised against synthetic peptide from human LHX1.
Epitope
aa149-165
Specificity
In human muscle, a 60 kD band is observed.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About LHX1
P48742 NM_005568 NP_005559.2

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Immunofluorescent analysis of LHX1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

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Images

Western blot

Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.
Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.

Western blot

Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.
Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.

Western blot

Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.
Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.

Western blot

Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.
Western blot of LIM-1 in muscle lysate with antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kD was detected.

Requested From: United States
Date Requested: 12/14/2018
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