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Catalog Number Size Price
LS-C744793-25 25 µl (1 mg/ml) $304 
LDHA / LDH1 Antibody - Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
LDHA / LDH1 Antibody - Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
LDHA / LDH1 Antibody - Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
LDHA / LDH1 Antibody - Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
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Polyclonal Goat anti‑Rabbit LDHA / LDH1 Antibody (Liquid, IF, WB) LS‑C744793

Polyclonal Goat anti‑Rabbit LDHA / LDH1 Antibody (Liquid, IF, WB) LS‑C744793

Antibody:
LDHA / LDH1 Goat anti-Rabbit Polyclonal (Liquid) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C744793-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LDHA / LDH1 Goat anti-Rabbit Polyclonal (Liquid) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Liquid

Specifications

Description
LDH1 antibody LS-C744793 is an unconjugated goat polyclonal antibody to rabbit LDH1 (LDHA). Validated for ELISA, IF, IP and WB.
Target
Rabbit LDHA / LDH1
Synonyms
LDHA | LDH muscle subunit | LDH1 | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Applications
  • Immunofluorescence (1:200)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:2000 - 1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LDHA / LDH1
P00338 NM_005566 NP_005557.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/26/2024