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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LASP1 Antibody LS‑C500099
LASP1 antibody LS-C500099 is an unconjugated rabbit polyclonal antibody to LASP1 from human and mouse. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C500099-50
50 µg
$285
LS-C500099-100
100 µg
$385

Popular LASP1 Products

Anti-LASP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
LASP1 antibody. IHC(P): Human Lung Cancer Tissue.
Species: Human, Monkey, Rat, Bovine, Pig, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, ICC, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
LASP1 antibody IHC-paraffin: Human Placenta Tissue.
Species: Human, Orangutan
Applications: IHC, IHC - Paraffin, Western blot

Product Description

LASP1 antibody LS-C500099 is an unconjugated rabbit polyclonal antibody to LASP1 from human and mouse. Validated for ELISA, IF, IHC and WB.

Specifications

Target
Human LASP1
Synonyms
LASP1, LIM and SH3 domain protein 1, MLN50, Lasp-1, LIM and SH3 protein 1, MLN 50
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LASP1 antibody was raised against recombinant human LIM and SH3 domain protein 1 protein (128-252AA)
Specificity
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LASP1
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types. Q14847 NM_006148 NP_006139.1


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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;
Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;
Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;
Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using LASP1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human gastric cancer using LASP1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using LASP1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;
Positive WB detected in:U87 whole cell lysate,HEK293 whole cell lysate,Jurkat whole cell lysate,Mouse lung tissue;All lanes: LASP1 antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,37,24 kDa;Observed band size: 30 kDa;

Requested From: United States
Date Requested: 11/16/2018

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