Human Laminin

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified laminin P1 fragment from human placenta

• IHC - Paraffin (1:250)
• Immunofluorescence (1:80)
• ELISA (1:200)
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. The antibody against Laminin primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion. Laminin is also found in extracellular matrix not related to basal membranes, e.g. in the proximity of neurons especially during development, regeneration and tumour development. Laminins are glycoproteins of the extracellular matrix, especially of the basal membranes. They are multi-functional molecules which play an important role during development and differentiation of organs and for cell movements. These properties rely on the interaction of laminins with receptors on cell surfaces like integrins, collagen IV, proteoglycans. Laminins consist of 2 heavy chains (A and M, 300-400 kDa) and 1 light chain (B1, B2 or S, 200 - 220 kDa). Human laminin fragment P1 100%, human collagen type I and IV <0.1%, human fibronectin <0.1% (RIA at 1:1000 dilution).

PBS

0.5 ml Reconstitute in sterile distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.