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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C152900-100 100 µg (1 mg/ml) $530 
Antibody

Laminin A / Laminin B Antibody LS‑C152900

Laminin A / Laminin B Antibody LS‑C152900

Antibody:
Rat Monoclonal to Mouse Laminin A / Laminin B
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$530 
LS-C152900-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse Laminin A / Laminin B
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Laminin B antibody LS-C152900 is an unconjugated rat monoclonal antibody to mouse Laminin B (Laminin A ). Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Target
Mouse Laminin A / Laminin B
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Laminin isolated from mouse EHS tumor.
Specificity
As original characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin 1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele et al (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kD 1/1 band and the 400 kD 1 band of mouse EHS laminin. However the affinity for the 400 kD band was very low. AL-4 also demonstrated strong binding the Laminin 1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin 1 chain, however the clone also recognizes the laminin 1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins 1 and 1, with the 1 reactivity being more prominent
Usage
ELISA/RIA. Western blot. Immunoprecipitation. Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.
Presentation
0.02 M PB, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
Storage
Store at 4°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (1)

The vestibular system is critical for the changes in muscle and bone induced by hypergravity in mice. Kawao N, Morita H, Obata K, Tamura Y, Okumoto K, Kaji H. Physiological reports. 2016 October;4:pii: e12979. (Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/16/2019