E. coli LacI / Lac Repressor

Rabbit

E. coli, S. sonnei (tested or 100% immunogen sequence identity)

S. sonnei (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.

This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.

• Western blot (1:500 - 1:2000)
• ELISA (1:90000 - 1:100000)

This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kD in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.