Target
Human KRT7 / CK7 / Cytokeratin 7
Synonyms
KRT7 | CK-7 | Cytokeratin 7 | CK7 | K2C7 | K7 | Keratin 7 | Keratin-7 | SCL | Type II mesothelial keratin K7 | Cytokeratin-7 | Sarcolectin | Type-II keratin Kb7
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Specificity
Recognizes human Cytokeratin 7 (exclusively). Reacts with a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- ICC
- Western blot (1:50 - 1:500)
- Flow Cytometry (1:50 - 1:100)
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Usage
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. This antibody reacts particularly well in paraffin embedded tissue. Flow Cytometry: 1:50-1:100. Immunohistochemistry: Frozen tissue after fixation in methanol. Paraffin embedded tissue sections pretreated with 0.1% pronase/ 0.1% trypsin in PBS or 0.5% pepsin in 0.01N HCl. Avidin-biotinylated horseradish peroxidase complex as a detection reagent. 1:50-1:100 (ABC). Western Blot: 1:50-1:500.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, 40% Glycerol, No stabilizing proteins added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT7 / CK7 / Cytokeratin 7