Human KLRB1 / CD161

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

B199.2

Unconjugated. Also available conjugated with FITC, Biotin, PE.

Protein A purified

Unmodified. Also available Preservative Free.

Activated human NK cells.

Antibody B199.2 recognizes the dimeric 160 kd CD161 molecule. In MLR, blockade with antibody B199.2 caused a slight but sigfnificant reduction in the percentage of IFN gamma-expressing NK cells challenged with M. tuberculosis

• Flow Cytometry

Five x 105 ficoll prepared human peripheral blood mononuclear cells per tube were pre incubated 5 minutes with 250 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD161 antibody at 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 22% sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q12918 NCBI: NM_002258 NP_002249.1