Human KLRB1 / CD161

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

B199.2

FITC. Also available Unconjugated or conjugated with Biotin, PE.

Protein A purified

Unmodified

Activated human NK cells.

Antibody B199.2 recognizes the dimeric 160 kd CD161 molecule. In MLR, blockade with antibody B199.2 caused a slight but sigfnificant reduction in the percentage of IFN gamma-expressing NK cells challenged with M. tuberculosis

• Flow Cytometry
• Applications tested for the base form of this product only

Five x 105 ficoll prepared human peripheral blood mononuclear cells per tube were pre incubated 5 minutes with 250 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of of anti-CD161/FITC at a 1:50 dilution (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.65 log10 fluorescent units when compared to a Mouse IgG2b/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD161 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q12918 NCBI: NM_002258 NP_002249.1