Mouse KIAA0319

Goat

Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-QGKIKQENKPTLH, from the internal region of the protein sequence according to NP_001074520.1.

aa391-403

Mouse KIAA0319. The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1), isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1).

• Western blot (0.5 - 1.5 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kD band observed in Mouse Brain lysates (calculated MW of 118kD according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15;17(6):859-71; PMID: 18063668). Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 25kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q5VV43 NCBI: NM_014809 NP_055624.2