Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C756523-10 10 µg $318 
LS-C756523-100 100 µg $470 
KCNN4 / KCa3.1 Antibody - Western blot analysis of KCNN4 using anti-KCNN4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysate,Lane 2: human PC-3 whole cell lysate,Lane 3: human A549 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: rat stomach tissue lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48KD. The expected band size for KCNN4 is at 48KD.

Polyclonal Rabbit anti‑Human KCNN4 / KCa3.1 Antibody (WB) LS‑C756523

Polyclonal Rabbit anti‑Human KCNN4 / KCa3.1 Antibody (WB) LS‑C756523

Antibody:
KCNN4 / KCa3.1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756523-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KCNN4 / KCa3.1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
KCa3.1 antibody LS-C756523 is an unconjugated rabbit polyclonal antibody to KCa3.1 (KCNN4) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human KCNN4 / KCa3.1
Synonyms
KCNN4 | HIKCa1 | HKCa4 | HIK1 | IK1 | KCA4 | HSK4 | IKCA1 | KCa3.1 | SK4 | SKCa 4 | Putative Gardos channel | SKCa4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human KCNN4 recombinant protein (Position: K309-Q364).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KCNN4 / KCa3.1
O15554 NM_002250 NP_002241.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2024