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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KARS Antibody (C‑Terminus) LS‑C287504
KARS antibody LS-C287504 is an unconjugated rabbit polyclonal antibody to human KARS. Validated for ICC, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C287504-100
100 µl (0.2 mg/ml)
$395

Popular KARS Products

Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8077 dilution 5-10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Immunohistochemistry of Human colon cancer using KARS Polyclonal Antibody at dilution of 1:60.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

KARS antibody LS-C287504 is an unconjugated rabbit polyclonal antibody to human KARS. Validated for ICC, IF, IHC, IP and WB.
About KARS
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Q15046 NM_005548 NP_005539.1

KARS Antibody, CMTRIB Antibody, KARS1 Antibody, KARS2 Antibody, KRS Antibody, Lysine--tRNA ligase Antibody, LysRS Antibody, KIAA0070 Antibody, Lysine tRNA ligase Antibody, Lysyl-tRNA synthetase Antibody


Specifications

Target
Human KARS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:50 - 1:250)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (1 - 4 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
C-Terminus
Specificity
Region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).
Usage
Immunohistochemistry: Antigen retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, K562 Cells, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.

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Images

Immunohistochemistry

Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.

Immunohistochemistry

Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.

Requested From: United States
Date Requested: 9/20/2018

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