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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KARS Antibody (C‑Terminus, HRP) LS‑C440699
KARS antibody LS-C440699 is an HRP-conjugated rabbit polyclonal antibody to KARS from human, mouse, chimpanzee and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C440699-100
100 µl (0.65 mg/ml)
$385
Description
KARS antibody LS-C440699 is an HRP-conjugated rabbit polyclonal antibody to KARS from human, mouse, chimpanzee and other species. Validated for IHC and WB.
Target
Human KARS
Host
Rabbit
Reactivity
Human, Chimpanzee, Orangutan, Monkey, Mouse, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Gibbon, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KARS antibody was raised against synthetic peptide from C-Terminus of human KARS (Q15046, NP_005539). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon, Bovine (92%); Horse (87%); Marmoset, Bat, Rabbit, Pig (85%); Guinea pig (84%); Mouse (78%).
Epitope
C-Terminus
Specificity
Human KARS
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About KARS
Q15046 NM_005548 NP_005539.1

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Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
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Images

Immunohistochemistry

Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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