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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JAG1 / Jagged 1 Antibody (aa110‑125) LS‑C18929

Immunohistochemistry - Jagged-1 Antibody.
Immunofluorescence - Jagged-1 Antibody. Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.
Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.
Immunohistochemistry - Jagged-1 Antibody.
Immunofluorescence - Jagged-1 Antibody. Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.
Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.
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JAG1 / Jagged 1 Antibody (aa110‑125) LS‑C18929

Antibody:
Rabbit Polyclonal to Human JAG1 / Jagged 1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$475 
LS-C18929-500
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human JAG1 / Jagged 1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Jagged 1 antibody LS-C18929 is an unconjugated rabbit polyclonal antibody to Jagged 1 (JAG1) from human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human JAG1 / Jagged 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
JAG1 / Jagged 1 antibody was raised against synthetic peptide from human JAG1 / Jagged 1.
Epitope
aa110-125
Specificity
Amino acids 110-125 of human Jagged-1protein.
Usage
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kD band in human brain and kidney lysates.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About JAG1 / Jagged 1
P78504 NM_000214 NP_000205.1

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Requested From: United States
Date Requested: 7/23/2019
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