Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C756617-10	10 µg	$318
LS-C756617-100	100 µg	$470

ITGB2 / CD18 Antibody - IHC analysis of CD18 using anti-CD18 antibody. CD18 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD18 antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ITGB2 / CD18 Antibody - IHC analysis of CD18 using anti-CD18 antibody. CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-CD18 antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

ITGB2 / CD18 Antibody - Western blot analysis of CD18 using anti-CD18 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD18 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

1 of 3

2 of 3

3 of 3

Monoclonal Mouse anti‑Human ITGB2 / CD18 Antibody (IHC, WB) LS‑C756617

Name: Anti-ITGB2 / CD18 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756617-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

ITGB2 / CD18 Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C756617-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ITGB2 / CD18 Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CD18 antibody LS-C756617 is an unconjugated mouse monoclonal antibody to human CD18 (ITGB2). Validated for Flow, ICC, IHC and WB.

Target

Human ITGB2 / CD18

Synonyms

ITGB2 | CD18 antigen | CD18 | Integrin beta chain, beta 2 | LCAMB | MAC-1 | MFI7 | MF17 | Integrin beta-2 | LAD | LFA-1

See All ITGB2 / CD18 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
IHC - Frozen (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
Flow Cytometry (1 - 3 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.