Target
Mouse IRS4
Synonyms
IRS4 | Insulin receptor substrate 4 | Phosphoprotein of 160 kDa | IRS-4 | Pp160 | PY160
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DRGTKERKEAKEVRD, from the internal region of the protein sequence according to NP_034702.2.
Applications
- Immunofluorescence
- Western blot (0.2 - 0.6 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 150kD band observed in Mouse Heart lysates (calculated MW of 130kD according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6 ug/ml. An additional band of unknown identity was also consistently observed at ~39kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee