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| Catalog Number | Size | Price |
|---|---|---|
| LS-C8274-100 | 100 µg | $973 |
Polyclonal Rabbit anti‑Human IRS1 Antibody (phospho‑Ser307, WB) LS‑C8274
Polyclonal Rabbit anti‑Human IRS1 Antibody (phospho‑Ser307, WB) LS‑C8274
Antibody:
IRS1 Rabbit anti-Human Polyclonal (pSer307) Antibody
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IRS1 Rabbit anti-Human Polyclonal (pSer307) Antibody
Application:
WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
IRS1 antibody LS-C8274 is an unconjugated rabbit polyclonal antibody to IRS1 (pSer307) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human IRS1
Synonyms
IRS1 | IRS-1 | HIRS-1 | Insulin receptor substrate 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa 298-316 of Human IRS-1 at 180kD. Peptide Sequence: GLTRRSRTE-pS-ITATSPASM.
Epitope
pSer307
Specificity
Recognizes human IRS-1, pS307. Minimal reactivity with non phosphorylated Ser307. Species sequence homology: mouse, rat, canine, vervet monkey 100%, porcine, bovine -94%, chicken 88%.
Applications
- Western blot (1:250 - 1:1500)
- ELISA (1:2000 - 1:4000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:2000-1:4000; Assayed against 0.1 ug of the immunizing peptide. Western Blot: 1:250-1:1500.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IRS1
Publications (0)
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human, Rat
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications:
ICC, Western blot, Immunoprecipitation
![IRS1 Antibody - Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/irs1-antibody-phospho-ser307-ls-c18824/62832_802708.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
