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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRS1 Antibody (Mouse phospho‑Ser302 and Human pSer307, Biotin) LS‑C527316
IRS1 antibody LS-C527316 is a biotin-conjugated mouse monoclonal antibody to IRS1 from human, mouse and rat. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C527316-100
100 µl
$890
Description
IRS1 antibody LS-C527316 is a biotin-conjugated mouse monoclonal antibody to IRS1 from human, mouse and rat. Validated for IF, IHC, IP and WB.
Target
Mouse IRS1
Host
Mouse
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with AP, APC, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
Mouse pSer302 and Human pSer307
Specificity
Recognizes IRS1 phosphorylated at Ser302 (mouse) and Ser307 (human). Species cross-reactivity: rat. Species sequence homology: monkey, bovine, canine, porcine. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.
About IRS1
P35568 NM_005544 NP_005535.1

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Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Species: Human
Applications: Western blot, ELISA

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Requested From: United States
Date Requested: 12/12/2018
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