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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRAK1 / IRAK Antibody LS‑C249

Catalog Number Size Price
LS-C249-0.1 0.1 mg (1 mg/ml) $365 
Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 µg/ml.
Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 ug/ml.
Western blot of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody at 0.5 ug/ml.
Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 µg/ml.
Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 ug/ml.
Western blot of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody at 0.5 ug/ml.
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IRAK1 / IRAK Antibody LS‑C249

Antibody:
Rabbit Polyclonal to Human IRAK1 / IRAK
Application:
ICC, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365 
LS-C249-0.1
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human IRAK1 / IRAK
Application:
ICC, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IRAK antibody LS-C249 is an unconjugated rabbit polyclonal antibody to IRAK (IRAK1 ) from human, mouse and rat. Validated for ELISA, ICC, IF, IP and WB.
Target
Human IRAK1 / IRAK
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Immunogen
a peptide corresponding to 13 amino acids near the C-terminus of human IRAK. The immunogen is located within the last 50 amino acids of IRAK.
Blocking Peptide
LS-E29830 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-C249. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
Usage
IRAK antibody can be used for Western blot at 1 ug/mL and for immunoprecipitation with 2 to 4 ug per sample. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 78 kDa Observed: 80 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IRAK1 / IRAK
P51617 NM_001569 NP_001560.2

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Featured Products

Product
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Anti-IRAK1 / IRAK antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-IRAK1 / IRAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Mouse Irak1 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The Irak1 antibody detected the Irak1 protein (arrow).
Species: Mouse
Applications: Western blot, Flow Cytometry
Western blot analysis of IRAK1 (pT209) expression in MCF7 IL1b-treated (A); K562 (B); MCF7 (C); Raw264.7 IL1b-treated (D) whole cell lysates.
Species: Human, Mouse
Applications: Western blot
Anti-IRAK1 / IRAK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Requested From: United States
Date Requested: 8/24/2019
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