Mouse Integrin AlphaV Beta6

Mouse

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Immunization of beta 6 knockout mice with wild type murine keratinocytes.

Recognizes mouse integrin alpha V beta 6. Blocks activity against mouse and human alpha V beta 6. Does not recognize alpha V or other alpha V integrins. Species cross-reactivity: Human, rat and mouse. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species.

• Immunoprecipitation
• Flow Cytometry
• Functional Assay

• IHC
• Western blot

Suitable for use in Flow Cytometry, Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Flow Cytometry (effective for mouse and human): Use buffers containing 1 mM Mg, since the epitope recognized is divalent cation-dependent. Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Function blocking: Inhibition of alpha V beta 6-mediated cell adhesion and cell migration are achieved at a concentration of 100 ug/ml using beta 6 transfected cells tested against TGFbeta LAP. Note: Cell lines adhere to simple ligands (e. g. fibronectin) using more integrins than alphav/beta6, so it would be necessary to also block other integrins (e. g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.

PBS, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.