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Catalog Number Size Price
LS-C662281-10 10 µg $318 
LS-C662281-100 100 µg $470 
IL17B Antibody - Western blot analysis of IL17B using anti-IL17B antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
IL17B Antibody - Western blot - Anti-IL17B Picoband Antibody
IL17B Antibody - Western blot analysis of IL17B using anti-IL17B antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
IL17B Antibody - Western blot - Anti-IL17B Picoband Antibody
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Polyclonal Rabbit anti‑Human IL17B Antibody (WB) LS‑C662281

Polyclonal Rabbit anti‑Human IL17B Antibody (WB) LS‑C662281

Antibody:
IL17B Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662281-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL17B Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL17B antibody LS-C662281 is an unconjugated rabbit polyclonal antibody to human IL17B. Validated for ELISA and WB.
Target
Human IL17B
Synonyms
IL17B | Cytokine Zcyto7 | Cytokine-like protein ZCYTO7 | IL-17B | Interleukin 17B | Interleukin 20 | Interleukin-17 beta | Interleukin-17B | NIRF | Interleukin-20 | ZCYTO7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IL17B recombinant protein (Position: Q21-F180).
Specificity
Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17B
Q9UHF5 NM_014443 NP_055258.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/3/2024