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Catalog Number Size Price
LS-C70951-100 100 µg $941 
Antibody

Polyclonal Goat anti‑Mouse IL15RA Antibody (Extracellular Domain, WB) LS‑C70951

Polyclonal Goat anti‑Mouse IL15RA Antibody (Extracellular Domain, WB) LS‑C70951

Antibody:
IL15RA Goat anti-Mouse Polyclonal (Extracellular Domain) Antibody
Application:
WB, Flo, ELISA, Func, Neut
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$941
LS-C70951-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL15RA Goat anti-Mouse Polyclonal (Extracellular Domain) Antibody
Application:
WB, Flo, ELISA, Func, Neut
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL15RA antibody LS-C70951 is an unconjugated goat polyclonal antibody to mouse IL15RA (Extracellular Domain). Validated for ELISA, Flow, Func, Neut and WB.
Target
Mouse IL15RA
Synonyms
IL15RA | CD215 antigen | IL-15RA | IL-15 receptor subunit alpha | IL-15R-alpha | Interleukin 15 receptor, alpha | CD215
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
NS0-derived rmIL-15Ra extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes mouse IL-15Ra.
Applications
  • Western blot
  • Flow Cytometry
  • ELISA
  • Functional Assay
  • Neutralization
Usage
Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 ug/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5% cross-reactivity with rhIL-15 R and less than 1% cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 ug/mL/1E6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Phosphate Buffered Saline (PBS)
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL15RA
Q13261 NM_002189 NP_002180.1

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Requested From: United States
Date Requested: 6/15/2024