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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL10RA Antibody (aa147‑175) IHC‑plus™ LS‑B15873
Note: This antibody replaces LS-C165169
IL10RA antibody LS-B15873 is an unconjugated rabbit polyclonal antibody to human IL10RA. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B15873-200
200 µl
$425
Description
IL10RA antibody LS-B15873 is an unconjugated rabbit polyclonal antibody to human IL10RA. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IL10RA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9767 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B15873. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa147-175
Specificity
This IL10RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human IL10RA.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About IL10RA
Q13651 NM_001558 NP_001549.2

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Images

Immunohistochemistry - Paraffin

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.
Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.

Flow Cytometry

IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.
Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.

Flow Cytometry

IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.
Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.

Flow Cytometry

IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry - Paraffin

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunofluorescence

Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IL10RA Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.
Western blot of IL10RA Antibody in MCF-7 cell line lysates (35 ug/lane). IL10RA (arrow) was detected using the purified antibody.

Flow Cytometry

IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL10RA Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/18/2018
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