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Antibodies > IL-33 > LS-C344883
 
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Catalog Number Size Price
LS-C344883-50 50 µg $365 
LS-C344883-100 100 µg $449 
IL-33 Antibody - Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown color). B. Isotype control with IgG2a (negative control).
IL-33 Antibody - Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) at 1:2,000 dilution. 1). Recombinant human IL-33 (His tagged). . 2). Recombinant human IL-33 (FLAG-tagged). . 3). Recombinant mouse IL-33 (FLAG-tagged). . 4). Other protein (His tagged, negative control).
IL-33 Antibody - Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.
IL-33 Antibody - Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm.
IL-33 Antibody - Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown color). B. Isotype control with IgG2a (negative control).
IL-33 Antibody - Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) at 1:2,000 dilution. 1). Recombinant human IL-33 (His tagged). . 2). Recombinant human IL-33 (FLAG-tagged). . 3). Recombinant mouse IL-33 (FLAG-tagged). . 4). Other protein (His tagged, negative control).
IL-33 Antibody - Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.
IL-33 Antibody - Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm.
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Monoclonal Mouse anti‑Human IL‑33 Antibody (clone IL33305B, IHC, WB) LS‑C344883

Monoclonal Mouse anti‑Human IL‑33 Antibody (clone IL33305B, IHC, WB) LS‑C344883

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Antibody:
IL-33 Mouse anti-Human Monoclonal (IL33305B) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA, Func, Inhb
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$365
LS-C344883-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL-33 Mouse anti-Human Monoclonal (IL33305B) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA, Func, Inhb
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL-33 antibody LS-C344883 is an unconjugated mouse monoclonal antibody to human IL-33. Validated for ELISA, Func, IHC, Inhb, IP and WB.
Target
Human IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
IL33305B
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Recombinant human IL-33
Specificity
Recognizes human IL-33. Does not cross-react with mouse IL-33.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:5000)
  • Functional Assay
  • Inhibition
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
ELISA - Direct or indirect: 1:2000-1:5000. Functional Application - Blocking. Immunohistochemistry - Paraffin sections (1:50-1:200. Western blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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