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Antibodies > IL-1B / IL-1 Beta > LS-C661920
 
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Catalog Number Size Price
LS-C661920-10 10 µg $318 
LS-C661920-100 100 µg $470 
IL-1B / IL-1 Beta Antibody - Western blot analysis of IL1 beta using anti-IL1 beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 31KD. The expected band size for IL1 beta is at 31KD.
IL-1B / IL-1 Beta Antibody - Western blot - Anti-IL1 beta Picoband Antibody
IL-1B / IL-1 Beta Antibody - Western blot analysis of IL1 beta using anti-IL1 beta antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 31KD. The expected band size for IL1 beta is at 31KD.
IL-1B / IL-1 Beta Antibody - Western blot - Anti-IL1 beta Picoband Antibody
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Polyclonal Rabbit anti‑Mouse IL‑1B / IL‑1 Beta Antibody (WB) LS‑C661920

Polyclonal Rabbit anti‑Mouse IL‑1B / IL‑1 Beta Antibody (WB) LS‑C661920

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Antibody:
IL-1B / IL-1 Beta Rabbit anti-Mouse Polyclonal Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661920-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL-1B / IL-1 Beta Rabbit anti-Mouse Polyclonal Antibody
Application:
WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL-1 Beta antibody LS-C661920 is an unconjugated rabbit polyclonal antibody to mouse IL-1 Beta (IL-1B). Validated for WB.
Target
Mouse IL-1B / IL-1 Beta
Synonyms
IL1B | IL-1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of mouse IL1 beta (DPKQYPKKKMEKRFVFNKIEVKSKVEFESAE).
Specificity
Expressed in activated macrophages (at protein level).
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-1B / IL-1 Beta
P01584 NM_000576 NP_000567.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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