ELISA: To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with LifeSpan's Biotinylated Anti-Human Nanog (LS-B8225
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNanog. Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5-3 ng/lane, under either reducing or non-reducing conditions.