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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL‑1B / IL‑1 Beta Antibody LS‑C104726
Note: This antibody replaces LS-C54322
IL-1 Beta antibody LS-C104726 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104726-500
500 µg
$255
Description
IL-1 Beta antibody LS-C104726 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA and WB.
Target
Human IL-1B / IL-1 Beta
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.25 - 0.5 µg/ml)
  • ELISA (0.25 - 0.5 µg/ml)
Immunogen
E. coli derived recombinant Human IL-1beta.
Specificity
Human IL-1beta
Usage
ELISA: To detect hNanog by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with LifeSpan's Biotinylated Anti-Human Nanog (LS-B8225) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNanog. Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IL-1B / IL-1 Beta
P01584 NM_000576 NP_000567.1

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Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
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Applications: Western blot, ELISA

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Images

Western blot

Anti-Human IL-1ß Western Blot Reduced
Anti-Human IL-1ß Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Anti-Human IL-1ß Sandwich ELISA
Anti-Human IL-1ß Sandwich ELISA

Western blot

Anti-Human IL-1ß Western Blot Reduced
Anti-Human IL-1ß Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Anti-Human IL-1ß Sandwich ELISA
Anti-Human IL-1ß Sandwich ELISA

Western blot

Anti-Human IL-1ß Western Blot Reduced
Anti-Human IL-1ß Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Anti-Human IL-1ß Sandwich ELISA
Anti-Human IL-1ß Sandwich ELISA

Western blot

Anti-Human IL-1ß Western Blot Reduced
Anti-Human IL-1ß Western Blot Reduced

Enzyme-Linked Immunosorbent Assay

Anti-Human IL-1ß Sandwich ELISA
Anti-Human IL-1ß Sandwich ELISA

Requested From: United States
Date Requested: 11/18/2018
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