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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL‑1B / IL‑1 Beta Antibody (clone 2A8) LS‑C197510
IL-1 Beta antibody LS-C197510 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA, IF, IP and WB.
Catalog
Size
Price
LS-C197510-100
100 µg (1 mg/ml)
$355

Popular IL-1B / IL-1 Beta Products

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
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Species: Human
Applications: IHC, Immunoprecipitation, ELISA, Neutralization
Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Species: Human, Dog, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Antibody
Species: Sheep
Applications: Western blot, ELISA

Product Description

IL-1 Beta antibody LS-C197510 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA, IF, IP and WB.

Specifications

Target
Human IL-1B / IL-1 Beta
Synonyms
IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [2A8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
IL1B (AAH08678, aa170-269 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human IL1B / IL-1 Beta
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About IL-1B / IL-1 Beta
IL-1 Beta is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. P01584 NM_000576 NP_000567.1


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Images

Immunofluorescence

Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.

Immunoprecipitation

Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.

Immunoprecipitation

Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.

Immunoprecipitation

Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.

Immunoprecipitation

Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/16/2018

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