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IHCPlus™ WNT8A Antibody Internal Polyclonal Azide‑free HRP IHC LS‑A107997

Catalog Number Size Price
LS-A107997-100 100 µl $650 
Anti-WNT8A antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ WNT8A Antibody Internal Polyclonal Azide‑free HRP IHC LS‑A107997

Antibody:
Rabbit Polyclonal to Human WNT8A
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Horse
Format:
Horseradish Peroxidase, Azide-free
Other formats:
Price
Catalog Number
$650 
LS-A107997-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human WNT8A
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Bat, Horse
Format:
Horseradish Peroxidase, Azide-free
Other formats:

Specifications

Description
WNT8A antibody LS-A107997 is an HRP-conjugated rabbit polyclonal antibody to WNT8A from human, bat, horse and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human WNT8A
Host
Rabbit
Reactivity
Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with AP, APC, Biotin, FITC, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 14 amino acid peptide from internal region of human WNT8A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Elephant, Panda, Horse (100%); Bovine, Dog (93%); Rabbit (86%).
Blocking Peptide
LS-E29428 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A107997. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human WNT8A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A8519 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A8519 was determined to be 10-20 ug/ml.
Presentation
PBS
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT8A
Q9H1J5 NM_058244 NP_490645.1

Validation

Anti-WNT8A antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-WNT8A antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT8A antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 8/22/2019
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