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Catalog Number Size Price
LS-A8730-50 50 µg (1 mg/ml) $440 
WNT5B Antibody - Anti-WNT5B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC‑plus™ Polyclonal Rabbit anti‑Human WNT5B Antibody (Internal, IHC) LS‑A8730

IHC‑plus™ Polyclonal Rabbit anti‑Human WNT5B Antibody (Internal, IHC) LS‑A8730

Antibody:
WNT5B Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$440
LS-A8730-50

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
WNT5B Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
WNT5B antibody LS-A8730 is an unconjugated rabbit polyclonal antibody to human WNT5B (Internal). Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human WNT5B
Synonyms
WNT5B | Protein Wnt-5b | WNT-5B protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 14 amino acid peptide from internal region of human WNT5B. Percent identity with other species by BLAST analysis: Human, Orangutan, Gibbon, Monkey (100%); Gorilla, Marmoset (93%); Dog, Elephant, Horse, Rabbit, Opossum (86%).
Epitope
Internal
Specificity
Human WNT5B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT5B
Q9H1J7 NM_030775 NP_116031.1

Validation

WNT5B Antibody - Anti-WNT5B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT5B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT5B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ WNT5B Antibody (Internal) for IHC LS-A8730 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

Differential expression of canonical and non-canonical Wnt ligands in ameloblastoma. Siar CH, Nagatsuka H, Han PP, Buery RR, Tsujigiwa H, Nakano K, Ng KH, Kawakami T. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2012 Apr;41:332-9.

Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset. Maschietto M, Trapé AP, Piccoli FS, Ricca TI, Dias AA, Coudry RA, Galante PA, Torres C, Fahhan L, Lourenço S, Grundy PE, de Camargo B, de Souza S, Neves EJ, Soares FA, Brentani H, Carraro DM. Cell death & disease. 2011 2:e224. (IHC; Human)

Customer Reviews (0)


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Requested From: United States
Date Requested: 8/3/2021