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Catalog Number Size Price
LS-B14853-50 50 µl $460 
WAS / WASP Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
WAS / WASP Antibody - Immunohistochemistry of paraffin-embedded rat kidney using WAS antibody at dilution of 1:100 (200x lens).
WAS / WASP Antibody - Immunohistochemistry of paraffin-embedded human esophagus using WAS antibody at dilution of 1:100 (200x lens).
WAS / WASP Antibody - Immunofluorescence analysis of U2OS cells using WAS antibody. Blue: DAPI for nuclear staining.
WAS / WASP Antibody - Western blot analysis of extracts of various cell lines, using WAS antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.
WAS / WASP Antibody - Western blot analysis of extracts of various cell lines, using WAS antibody.
WAS / WASP Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
WAS / WASP Antibody - Immunohistochemistry of paraffin-embedded rat kidney using WAS antibody at dilution of 1:100 (200x lens).
WAS / WASP Antibody - Immunohistochemistry of paraffin-embedded human esophagus using WAS antibody at dilution of 1:100 (200x lens).
WAS / WASP Antibody - Immunofluorescence analysis of U2OS cells using WAS antibody. Blue: DAPI for nuclear staining.
WAS / WASP Antibody - Western blot analysis of extracts of various cell lines, using WAS antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.
WAS / WASP Antibody - Western blot analysis of extracts of various cell lines, using WAS antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human WAS / WASP Antibody (IHC, IF, WB) LS‑B14853

IHC‑plus™ Polyclonal Rabbit anti‑Human WAS / WASP Antibody (IHC, IF, WB) LS‑B14853

Note: This antibody replaces LS-C331179
Antibody:
WAS / WASP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14853-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
WAS / WASP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
WASP antibody LS-B14853 is an unconjugated rabbit polyclonal antibody to human WASP (WAS). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human WAS / WASP
Synonyms
WAS | Eczema-thrombocytopenia | IMD2 | SCNX | WASP | THC | THC1 | Wiskott-Aldrich syndrome | Thrombocytopenia 1 (X-linked)
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WAS (NP_000368.1). GAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHV
Specificity
Human WAS / WASP
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 52kDa, while the observed MW by Western blot was 62kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WAS / WASP
P42768 NM_000377 NP_000368.1

Validation

WAS / WASP Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ WAS / WASP Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B14853 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024