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Catalog Number Size Price
LS-B11251-50 50 µg (2.814 mg/ml) $460 
VTCN1 / B7-H4 Antibody - Anti-VTCN1 / B7-H4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
VTCN1 / B7-H4 Antibody - Immunofluorescence staining of Hela cells with VTCN1 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
VTCN1 / B7-H4 Antibody - Anti-VTCN1 / B7-H4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
VTCN1 / B7-H4 Antibody - Immunofluorescence staining of Hela cells with VTCN1 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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IHC‑plus™ Polyclonal Rabbit anti‑Human VTCN1 / B7‑H4 Antibody (IHC, WB) LS‑B11251

IHC‑plus™ Polyclonal Rabbit anti‑Human VTCN1 / B7‑H4 Antibody (IHC, WB) LS‑B11251

Note: This antibody replaces LS-C211325
Antibody:
VTCN1 / B7-H4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-B11251-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
VTCN1 / B7-H4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
B7-H4 antibody LS-B11251 is an unconjugated rabbit polyclonal antibody to human B7-H4 (VTCN1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human VTCN1 / B7-H4
Synonyms
VTCN1 | B7 superfamily member 1 | B7 Superfamily, member 1 | B7h.5 | B7S1 | B7H4 | PRO1291 | VCTN1 | RP11-229A19.4 | B7 family member, H4 | B7-H4 | B7X | Protein B7S1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant protein encoding amino acids 26-258 of Uniprot Q7Z7D3
Specificity
Human VTCN1
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VTCN1 / B7-H4
Q7Z7D3 NM_024626 NP_078902.2

Validation

VTCN1 / B7-H4 Antibody - Anti-VTCN1 / B7-H4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-VTCN1 / B7-H4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-VTCN1 / B7-H4 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ VTCN1 / B7-H4 Antibody for IHC, WB/Western, ELISA LS-B11251 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024