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IHCPlus™ TXNDC5 / ERP46 Antibody aa269‑282 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9232

Catalog Number Size Price
LS-B9232-50 50 µg (0.5 mg/ml) $425 
Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-TXNDC5 (aa269-282) Antibody (0.1µg/ml) staining of Mouse Lymph Nodes (A), Stomach (B) and Intestine (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescencence.
TXNDC5 antibody (0.05 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-TXNDC5 (aa269-282) Antibody (0.1µg/ml) staining of Mouse Lymph Nodes (A), Stomach (B) and Intestine (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescencence.
TXNDC5 antibody (0.05 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ TXNDC5 / ERP46 Antibody aa269‑282 Polyclonal IHC,Peptide‑ELISA,WB LS‑B9232

Note: This antibody replaces LS-C139775
Antibody:
Goat Polyclonal to Human TXNDC5 / ERP46
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B9232-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human TXNDC5 / ERP46
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
ERP46 antibody LS-B9232 is an unconjugated goat polyclonal antibody to ERP46 (TXNDC5 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TXNDC5 / ERP46
Host
Goat
Reactivity
Human, Mouse, Rat, Bovine, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-RDGKKVDQYKGKRD, from the internal region of the protein sequence according to NP_110437.2NP_001139021.1.
Blocking Peptide
LS-E27666 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9232. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa269-282
Specificity
Human TXNDC5. This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50+38kD bands observed in Human Lymph Nodes lysates (calculated MW of 47.6kD according to NP_110437.2 and of 36.2kD according to NP_001139021.1). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TXNDC5 / ERP46
Q8NBS9 NM_030810 NP_110437.2

Validation

Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TXNDC5 / ERP46 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 9/16/2019