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Antibodies > TOP2A / Topoisomerase II Alpha > LS-B16084
 
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Catalog Number Size Price
LS-B16084-50 50 µl (1 mg/ml) $460 
TOP2A / Topoisomerase II Alpha Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TOP2A / Topoisomerase II Alpha Antibody - Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TOP2A / Topoisomerase II Alpha Antibody - Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.
TOP2A / Topoisomerase II Alpha Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TOP2A / Topoisomerase II Alpha Antibody - Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TOP2A / Topoisomerase II Alpha Antibody - Western blot analysis of Topoisomerase 2 alpha expression in MDAMB468 (A); MDAMB231 (B); HeLa (C) whole cell lysates.
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IHC‑plus™ Polyclonal Rabbit anti‑Human TOP2A / Topoisomerase II Alpha Antibody (N‑Terminus, IHC, IF, WB) LS‑B16084

IHC‑plus™ Polyclonal Rabbit anti‑Human TOP2A / Topoisomerase II Alpha Antibody (N‑Terminus, IHC, IF, WB) LS‑B16084

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C352956
Antibody:
TOP2A / Topoisomerase II Alpha Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16084-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TOP2A / Topoisomerase II Alpha Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Topoisomerase II Alpha antibody LS-B16084 is an unconjugated rabbit polyclonal antibody to human Topoisomerase II Alpha (TOP2A) (N-Terminus). Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TOP2A / Topoisomerase II Alpha
Synonyms
TOP2A | DNA topoisomerase II, 170 kD | TOP2 | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Topoisomerase 2 alpha.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of Topoisomerase 2 alpha protein.
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TOP2A / Topoisomerase II Alpha
P11388 NM_001067 NP_001058.2

Validation

TOP2A / Topoisomerase II Alpha Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ TOP2A / Topoisomerase II Alpha Antibody (N-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B16084 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

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Customer Reviews (0)

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Featured Products

TOP2A / Topoisomerase II Alpha Antibody - Anti-TOP2A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ TOP2A / Topoisomerase II Alpha Antibody (aa1‑50) LS‑B7670
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
TOP2A / Topoisomerase II Alpha Antibody (C‑Terminus) LS‑C26364
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
TOP2A / Topoisomerase II Alpha Antibody - IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOP2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
TOP2A / Topoisomerase II Alpha Antibody (aa1100‑1531, clone OTI5E6) LS‑C336588
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TOP2A Antibody. The picture on the right is blocked with the synthesized peptide.
TOP2A / Topoisomerase II Alpha Antibody (aa1482‑1531) LS‑C199673
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
TOP2A / Topoisomerase II Alpha Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ TOP2A / Topoisomerase II Alpha Antibody (aa1100‑1531, clone 1D2) LS‑B13281
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody. heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody (aa1100‑1531, clone UMAB145) LS‑C796865
Species: Human
Applications: IHC, Immunofluorescence, Western blot
See all TOP2A / Topoisomerase II Alpha Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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