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IHCPlus™ THRA / THR Alpha Antibody Ligand‑binding Domain Polyclonal Biotin IHC LS‑A106135

Catalog Number Size Price
LS-A106135-100 100 µl $650 
Anti-THRA antibody IHC of human surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ THRA / THR Alpha Antibody Ligand‑binding Domain Polyclonal Biotin IHC LS‑A106135

Antibody:
Rabbit Polyclonal to Human THRA / THR Alpha
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A106135-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human THRA / THR Alpha
Application:
IHC, IHC-P
Reactivity:
Human, Monkey
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
THR Alpha antibody LS-A106135 is a biotin-conjugated rabbit polyclonal antibody to THR Alpha (THRA ) from human and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human THRA / THR Alpha
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with AP, APC, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from ligand-binding domain of human THRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Sheep, Dog, Bovine, Hamster, Elephant, Panda, Rabbit, Horse, Pig (95%); Lizard (85%); Opossum (80%).
Blocking Peptide
LS-E29114 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A106135. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Ligand-binding Domain
Specificity
Human THRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A6194 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A6194 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THRA / THR Alpha
P10827 NM_003250 NP_003241.2

Validation

Anti-THRA antibody IHC of human surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-THRA antibody IHC of human surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-THRA antibody IHC of human surface epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 9/22/2019