Rat STX12 / Syntaxin 12

Mouse

Rat, Human, Mouse, Dog, Hamster (tested or 100% immunogen sequence identity)

IgG Monoclonal

15G2

Unconjugated

Protein G purified

Unmodified

Recombinant cytoplasmic domain of rat syntaxin 13.

Cytoplasmic Domain

Identifies recombinant rat syntaxin 13 and will detect an ~38 kD protein, corresponding to the apparent molecular mass of syntaxin 13 on SDS-PAGE immunoblots, in samples from mouse, rat, dog and hamster origins. Additional bands of 27 and 37 kD may also be recognized in these lysates, and are believed to correspond to degradation products of syntaxin 13.

• IHC
• IHC - Paraffin (20 µg/ml)
• Western blot (1 µg/ml)

Immunohistochemistry: LS-B2989 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2989 was determined to be 20 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86Y82 NCBI: NM_177424 NP_803173.1