LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

IHCPlus™ SMARCA4 / BRG1 Antibody aa75‑125 Polyclonal IHC,IP,WB LS‑B8447

Catalog Number Size Price
LS-B8447-50 50 µl (0.2 mg/ml) $485 
Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHCPlus™ SMARCA4 / BRG1 Antibody aa75‑125 Polyclonal IHC,IP,WB LS‑B8447

Note: This antibody replaces LS-C89131
Antibody:
Rabbit Polyclonal to Human SMARCA4 / BRG1
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B8447-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human SMARCA4 / BRG1
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BRG1 antibody LS-B8447 is an unconjugated rabbit polyclonal antibody to BRG1 (SMARCA4 ) from human and mouse. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SMARCA4 / BRG1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to amino acid residues 75 and 125 of human BRM/SWI2-Related Gene 1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily A, member 4) using the numbering given in entry NP_003063.2 (GeneID 6597).
Epitope
aa75-125
Specificity
Species cross-reactivity: Human and mouse. Other species have not been tested.
Usage
Suitable for use in Western Blot, Immunohistochemistry with FFPE tissues and Immunoprecipitation. Immunoprecipitation: 2-5 ug/mg lysate. Western Blot: 1:2000-1:10000.
Presentation
TBS, pH 7.2, 0.09% Sodium Azide, 0.1% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMARCA4 / BRG1
P51532 NM_003072 NP_003063.2

Validation

Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC Validated
Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Publications (1)

Transcription factor Foxp3 and its protein partners form a complex regulatory network. Rudra D, deRoos P, Chaudhry A, Niec RE, Arvey A, Samstein RM, Leslie C, Shaffer SA, Goodlett DR, Rudensky AY. Nature immunology. 2012 Oct;13:1010-9. (ICC, WB; Human)

Reviews (0)

Featured Products

Anti-SMARCA4 / BRG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Anti-SMARCA4 / BRG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-SMARCA4 mouse mAb (dilution 1:50). DAPI was used to stain nucleus(blue).
Species: Human, Mouse
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Detection of Mouse BRG1 by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRG1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation

Requested From: United States
Date Requested: 8/22/2019
Get Social With Us!
Follow us on Facebook Follow us on LinkedIn
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy