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IHCPlus™ SMAD3 Antibody Monoclonal ELISA,IHC,IP,WB LS‑B2555

Catalog Number Size Price
LS-B2555-50 50 µl (0.5 mg/ml) $425 
Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot Image
Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Western blot Image
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IHCPlus™ SMAD3 Antibody Monoclonal ELISA,IHC,IP,WB LS‑B2555

Note: This antibody replaces LS-C62161
Antibody:
Mouse Monoclonal to Human SMAD3
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2555-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human SMAD3
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD3 antibody LS-B2555 is an unconjugated mouse monoclonal antibody to SMAD3 from human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SMAD3
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
AF9F7
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human protein purified from E. coli (His-Smad3)
Usage
Immunohistochemistry: LS-B2555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2555 was determined to be 1:50.
Presentation
HEPES, 0.15 M NaCl, 0.03% Sodium Azide, 50% Glycerol, 0.01% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD3
P84022 NM_005902 NP_005893.1

Validation

Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC Validated
Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-SMAD3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

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Requested From: United States
Date Requested: 9/20/2019