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Catalog Number Size Price
LS-B10468-50 50 µg (0.5 mg/ml) $485 
SH3GL1 / EEN Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
SH3GL1 / EEN Antibody - SH3GL1 antibody (0.3 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SH3GL1 / EEN Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
SH3GL1 / EEN Antibody - SH3GL1 antibody (0.3 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHC‑plus™ Polyclonal Goat anti‑Human SH3GL1 / EEN Antibody (aa261‑272, IHC, WB) LS‑B10468

IHC‑plus™ Polyclonal Goat anti‑Human SH3GL1 / EEN Antibody (aa261‑272, IHC, WB) LS‑B10468

Note: This antibody replaces LS-C139731
Antibody:
SH3GL1 / EEN Goat anti-Human Polyclonal (aa261-272) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B10468-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SH3GL1 / EEN Goat anti-Human Polyclonal (aa261-272) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
EEN antibody LS-B10468 is an unconjugated goat polyclonal antibody to EEN (SH3GL1) (aa261-272) from human. It is reactive with human, dog and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SH3GL1 / EEN
Synonyms
SH3GL1 | CNSA1 | Endophilin-A2 | Endophilin-2 | Extra 11-19 leukemia fusion | SH3-domain GRB2-like 1 | SH3 domain protein 2B | EEN | EEN fusion partner of MLL | SH3D2B | SH3P8
Host
Goat
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EPFDLGEPEQSN, from the internal region of the protein sequence according to NP_003016.1.
Epitope
aa261-272
Specificity
Human SH3GL1 / Endophilin-A2.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in lysates of cell line K562 (calculated MW of 41.5kD according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SH3GL1 / EEN
Q99961 NM_003025 NP_003016.1

Validation

SH3GL1 / EEN Antibody - Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Human, Small intestine: Formalin-Fixed Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ SH3GL1 / EEN Antibody (aa261-272) for IHC, WB/Western LS-B10468 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/2/2024