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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B2301-50 50 µg (0.5 mg/ml) $425 
ROBO2 Antibody - Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ Polyclonal Goat anti‑Human ROBO2 Antibody (aa1281‑1292, IHC) LS‑B2301

IHCPlus™ Polyclonal Goat anti‑Human ROBO2 Antibody (aa1281‑1292, IHC) LS‑B2301

Note: This antibody replaces LS-C55417
Antibody:
Goat Polyclonal to Human ROBO2
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2301-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human ROBO2
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ROBO2 antibody LS-B2301 is an unconjugated goat polyclonal antibody to ROBO2 (aa1281-1292) from human. It is reactive with human, mouse and rat. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human ROBO2
Synonyms
ROBO2 | KIAA1568 | Roundabout homolog 2 | SAX3
Host
Goat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-NQSQRPRPTKKHK from an internal region (near the C-Terminus) of human ROBO2 (NP_002933.1). Percent identity by BLAST analysis: Mouse, Rat, Opossum (100%); Human, Gorilla, Monkey, Marmoset, Elephant, Panda, Bovine, Bat, Pig, Chicken (92%); Dog, Rabbit (85%).
Blocking Peptide
LS-E26551 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2301. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1281-1292
Specificity
Human ROBO2.
Usage
Immunohistochemistry: LS-B2301 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2301 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ROBO2
Q9HCK4 AF040991 AAC39576.1

Validation

ROBO2 Antibody - Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ROBO2 Antibody (aa1281-1292) for IHC LS-B2301 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 6/3/2020