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IHCPlus™ ROBO2 Antibody aa1281‑1292 Polyclonal IHC,Peptide‑ELISA LS‑B2301

Catalog Number Size Price
LS-B2301-50 50 µg (0.5 mg/ml) $425 
Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ ROBO2 Antibody aa1281‑1292 Polyclonal IHC,Peptide‑ELISA LS‑B2301

Note: This antibody replaces LS-C55417
Antibody:
Goat Polyclonal to Human ROBO2
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2301-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ROBO2
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ROBO2 antibody LS-B2301 is an unconjugated goat polyclonal antibody to ROBO2 from human, mouse and rat. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human ROBO2
Host
Goat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-NQSQRPRPTKKHK from an internal region (near the C-terminus) of human ROBO2 (NP_002933.1). Percent identity by BLAST analysis: Mouse, Rat, Opossum (100%); Human, Gorilla, Monkey, Marmoset, Elephant, Panda, Bovine, Bat, Pig, Chicken (92%); Dog, Rabbit (85%).
Blocking Peptide
LS-E26551 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2301. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1281-1292
Specificity
Human ROBO2.
Usage
Immunohistochemistry: LS-B2301 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2301 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ROBO2
Q9HCK4 AF040991 AAC39576.1

Validation

Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Anti-ROBO2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Zebrafish
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Anti-ROBO2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Reactivity: Rat
Range: 0.156-10 ng/ml
Reactivity: Rat
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Requested From: United States
Date Requested: 9/15/2019