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IHCPlus™ PTGER3 / EP3 Antibody Cytoplasmic Domain Polyclonal ELISA,IHC LS‑A973

Catalog Number Size Price
LS-A973-50 50 µg (1 mg/ml) $425 
Anti-PTGER3 / EP3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHCPlus™ PTGER3 / EP3 Antibody Cytoplasmic Domain Polyclonal ELISA,IHC LS‑A973

Antibody:
Rabbit Polyclonal to Human PTGER3 / EP3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A973-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PTGER3 / EP3
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
EP3 antibody LS-A973 is an unconjugated rabbit polyclonal antibody to EP3 (PTGER3 ) from human, mouse, rat and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human PTGER3 / EP3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human PTGER3 / EP3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bat, Panda, Horse, Rabbit (100%); Elephant, Pig (94%); Monkey, Bovine (89%).
Blocking Peptide
LS-E29744 - Liquid - 50 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-A973. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human PTGER3 / EP3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A973 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A973 was determined to be 4 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTGER3 / EP3
P43115 NM_000957 NP_000948.2

Validation

Anti-PTGER3 / EP3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-PTGER3 / EP3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PTGER3 / EP3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 8/19/2019
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