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Catalog Number Size Price
LS-B8331-50 50 µg (0.5 mg/ml) $485 
POU2AF1 / BOB1 Antibody - Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
POU2AF1 / BOB1 Antibody - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
POU2AF1 / BOB1 Antibody - Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
POU2AF1 / BOB1 Antibody - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
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IHC‑plus™ Polyclonal Goat anti‑Human POU2AF1 / BOB1 Antibody (aa241‑254, IHC, WB) LS‑B8331

IHC‑plus™ Polyclonal Goat anti‑Human POU2AF1 / BOB1 Antibody (aa241‑254, IHC, WB) LS‑B8331

Note: This antibody replaces LS-C139798
Antibody:
POU2AF1 / BOB1 Goat anti-Human Polyclonal (aa241-254) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B8331-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POU2AF1 / BOB1 Goat anti-Human Polyclonal (aa241-254) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
BOB1 antibody LS-B8331 is an unconjugated goat polyclonal antibody to BOB1 (POU2AF1) (aa241-254) from human. It is reactive with human, bat, horse and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human POU2AF1 / BOB1
Synonyms
POU2AF1 | BOB-1 | OBF-1 | OCT-binding factor 1 | BOB1 | OBF1 | OCA-B | OCAB
Host
Goat
Reactivity
Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-DSDAYALNHTLSVE, from the C-Terminus of protein sequence according to NP_006226.2.
Epitope
aa241-254
Specificity
Human POU2AF1 / BOB1.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POU2AF1 / BOB1
Q16633 NM_006235 NP_006226.2

Validation

POU2AF1 / BOB1 Antibody - Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

IHC-plus™ POU2AF1 / BOB1 Antibody (aa241-254) for IHC, WB/Western LS-B8331 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/7/2024