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Catalog Number Size Price
LS-B65-50 50 µg $485 
PDCD4 Antibody - Anti-PDCD4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PDCD4 Antibody - Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.
PDCD4 Antibody - Anti-PDCD4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PDCD4 Antibody - Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD4 Antibody (IHC, WB) LS‑B65

IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD4 Antibody (IHC, WB) LS‑B65

Note: This antibody replaces LS-C18980, LS-C93126
Antibody:
PDCD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B65-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDCD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
PDCD4 antibody LS-B65 is an unconjugated rabbit polyclonal antibody to PDCD4 from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PDCD4
Synonyms
PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a
Host
Rabbit
Reactivity
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000 - 1:10000)
  • ELISA (1:10000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B65 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B65 was determined to be 10 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD4
Q53EL6 NM_014456 NP_055271.2

Validation

PDCD4 Antibody - Anti-PDCD4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDCD4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-PDCD4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ PDCD4 Antibody for IHC, WB/Western, ELISA LS-B65 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022