Synthetic 17 amino acid peptide near C-terminus of human NR5A1. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Rabbit, Opossum (94%); Panda, Horse, Pig, Turkey, Chicken (88%); Platypus (82%).
Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A5338 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A5338 was determined to be 3.2 ug/ml.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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