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IHCPlus™ NFI / NFIC Antibody aa323‑372 Polyclonal IHC,WB LS‑B4029

Catalog Number Size Price
LS-B4029-100 100 µl (0.5 mg/ml) $485 
Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ NFI / NFIC Antibody aa323‑372 Polyclonal IHC,WB LS‑B4029

Note: This antibody replaces LS-C102863
Antibody:
Rabbit Polyclonal to Human NFI / NFIC
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B4029-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NFI / NFIC
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NFIC antibody LS-B4029 is an unconjugated rabbit polyclonal antibody to NFIC (NFI ) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human NFI / NFIC
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%); Zebra finch, Chicken (93%); Opossum (92%); Xenopus (86%); Stickleback (80%).
Blocking Peptide
LS-E10798 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4029. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa323-372
Specificity
Human NFIC
Usage
Immunohistochemistry: LS-B4029 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4029 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFI / NFIC
P08651 NM_005597 NP_005588.2

Validation

Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

Bone morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium. Rakian A, Yang WC, Gluhak-Heinrich J, Cui Y, Harris MA, Villarreal D, Feng JQ, Macdougall M, Harris SE. International journal of oral science. 2013 5:75-84. (WB; Human)

Reviews (0)

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Requested From: United States
Date Requested: 9/22/2019