Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B6433-50 50 µg (1 mg/ml) $485 
MKI67 / Ki67 Antibody - Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC‑plus™ Monoclonal Mouse anti‑Human MKI67 / Ki67 Antibody (clone Ki‑S5, IHC, WB) LS‑B6433

IHC‑plus™ Monoclonal Mouse anti‑Human MKI67 / Ki67 Antibody (clone Ki‑S5, IHC, WB) LS‑B6433

Note: This antibody replaces LS-C25007
Antibody:
MKI67 / Ki67 Mouse anti-Human Monoclonal (Ki-S5) Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B6433-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MKI67 / Ki67 Mouse anti-Human Monoclonal (Ki-S5) Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Ki67 antibody LS-B6433 is an unconjugated mouse monoclonal antibody to human Ki67 (MKI67). Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human MKI67 / Ki67
Synonyms
MKI67 | Antigen KI-67 | Ki67 | KIA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
Ki-S5
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Specificity
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC (5 - 10 µg/ml)
  • Western blot (1:500)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MKI67 / Ki67
P46013 NM_002417 NP_002408.3

Validation

MKI67 / Ki67 Antibody - Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ MKI67 / Ki67 Antibody (clone Ki-S5) for IHC, ICC, WB/Western, Flow LS-B6433 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer. Kotaro Abe, Masahiko Kanehira , Shinya Ohkouchi, Sakiko Kumata, Yamato Suzuki, Hisashi Oishi, Masafumi Noda, Akira Sakurada, Eisaku Miyauchi, Tohru Fujiwara, Hideo Harigae, Yoshinori Okada. Cancer medicine. 2021 May;;10:3085-3100.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/21/2024