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Catalog Number Size Price
LS-B2925-50 50 µl (1 mg/ml) $460 
MIF Antibody - Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
MIF Antibody - The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
MIF Antibody - Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
MIF Antibody - The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
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IHC‑plus™ Monoclonal Mouse anti‑Human MIF Antibody (clone 4E4, IHC, WB) LS‑B2925

IHC‑plus™ Monoclonal Mouse anti‑Human MIF Antibody (clone 4E4, IHC, WB) LS‑B2925

Note: This antibody replaces LS-C93767
Antibody:
MIF Mouse anti-Human Monoclonal (4E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2925-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MIF Mouse anti-Human Monoclonal (4E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MIF antibody LS-B2925 is an unconjugated mouse monoclonal antibody to human MIF. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MIF
Synonyms
MIF | GLIF | L-dopachrome tautomerase | L-dopachrome isomerase | MMIF | Phenylpyruvate tautomerase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
4E4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2925 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2925 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MIF
P14174 NM_002415 NP_002406.1

Validation

MIF Antibody - Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ MIF Antibody (clone 4E4) for IHC, WB/Western, ELISA LS-B2925 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2024