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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ Melanoma‑Associated Antigen Antibody Monoclonal ELISA,IF,IHC,IP LS‑B7204

Catalog Number Size Price
LS-B7204-0.05 0.05 mg (1 mg/ml) $425 
Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ Melanoma‑Associated Antigen Antibody Monoclonal ELISA,IF,IHC,IP LS‑B7204

Note: This antibody replaces LS-C149086, LS-C150499
Antibody:
Mouse Monoclonal to Human Melanoma-Associated Antigen
Application:
IHC, IHC-P, IHC-Fr, IF, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7204-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Melanoma-Associated Antigen
Application:
IHC, IHC-P, IHC-Fr, IF, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Melanoma-Associated Antigen antibody LS-B7204 is an unconjugated mouse monoclonal antibody to human Melanoma-Associated Antigen. Validated for ELISA, IF, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Target
Human Melanoma-Associated Antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
NKI-C3
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Melanoma-associated antigen
Specificity
Used for diagnostic by staining of lysosomes/melanosomes. Directed against a predominantly intracellular protein of human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three V-linked glycans were added cotranslationally. Extensive further heterogeneity was generated in the Colgi compartmen and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with cathepsin D, which is a marker for lysosomal functions.This antibody was originally described as a marker for melanoma. Recently, it resurfaced as a marker to separate cellular neurothekeoma from other dermal tumors in the differential diagnosis.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Melanoma-Associated Antigen antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Range: 0.27-200 pg/ml

Requested From: United States
Date Requested: 8/21/2019
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